<?xml version="1.0" encoding="UTF-8"?>
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    <admin>
        <current_status>
            <date>2023-08-16</date>
            <code>OBS</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2021-08-16</deposition>
            <header_release>2023-02-22</header_release>
            <map_release>2023-02-22</map_release>
            <obsolete>2023-08-16</obsolete>
            <update>2023-08-16</update>
        </key_dates>
        <superseded_by_list>
            <entry>
                <date>2023-08-16</date>
                <entry>EMD-17595</entry>
            </entry>
        </superseded_by_list>
        <grant_support>
            <grant_reference>
                <funding_body>Grant Agency of the Czech Republic</funding_body>
                <code>GA19-14360S</code>
                <country>Czech Republic</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Ministry of Education (MoE, Czech Republic)</funding_body>
                <code>LM2018127</code>
                <country>Czech Republic</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Ministry of Education (MoE, Czech Republic)</funding_body>
                <code>LO1304</code>
                <country>Czech Republic</country>
            </grant_reference>
        </grant_support>
        <title>H3K36 tri-methylated nucleosome LEDGF PWWP domain complex pose 2</title>
        <authors_list>
            <author>Koutna E</author>
            <author>Lux V</author>
        </authors_list>
        <keywords>nucleosome, transcription activator, methylation, complex, DNA BINDING PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Koutna E</author>
                    <author order="2">Lux V</author>
                    <author order="3">Novacek J</author>
                    <author order="4">Srb P</author>
                    <author order="5">Hexnerova R</author>
                    <author order="6">Skerlova J</author>
                    <author order="7">Poepsel S</author>
                    <author order="8">Veverka V</author>
                    <title>Conservation of H3K36 di- and trimethylated nucleosome recognition by PWWP</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>7pgw</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>H3K36 tri-methylated nucleosome : LEDGF PWWP domain complex - pose 2</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>H3K36 tri-methylated nucleosome : LEDGF PWWP domain complex - pose 2</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Histone H3.2</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.015331982</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGV(ML3)KPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQ
DFKTDLRFQSSAVMALQEASEAYLVALFEDTNLAAIHAKRVTIMPKDIQLARRIRGERA</string>
                    <external_references type="UNIPROTKB">P84233</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Histone H4</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.011263230999999999</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKT
VTAMDVVYALKRQGRTLYGFGG</string>
                    <external_references type="UNIPROTKB">P62799</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Histone H2A</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.013978240999999999</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>SGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIP
RHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSK</string>
                    <external_references type="UNIPROTKB">Q6AZJ8</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="4">
                <name>Histone H2B 1.1</name>
                <natural_source database="NCBI">
                    <organism ncbi="8355">Xenopus laevis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.013524752</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>AKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHY
NKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK</string>
                    <external_references type="UNIPROTKB">P02281</external_references>
                </sequence>
            </protein_or_peptide>
            <dna macromolecule_id="5">
                <name>Wisdom 601 DNA</name>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
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                    <theoretical units="MDa">0.050699315999999994</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
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(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA)
(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)(DA)
(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)(DC)
(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG)
(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG)
(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)(DA)
(DT)(DC)(DC)(DT)(DG)(DT)(DG)(DC)(DA)(DT)(DG)(DT)(DA)(DT)(DT)(DG)(DA)(DA)(DC)(DA)
(DG)(DC)(DG)(DA)(DC)</string>
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            <dna macromolecule_id="6">
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                <number_of_copies>1</number_of_copies>
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                    <string>(DG)(DT)(DC)(DG)(DC)(DT)(DG)(DT)(DT)(DC)(DA)(DA)(DT)(DA)(DC)(DA)(DT)(DG)(DC)(DA)
(DC)(DA)(DG)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA)
(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG)
(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA)
(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)(DC)
(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)
(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)
(DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)
(DC)(DT)(DG)(DA)(DT)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <protein_or_peptide macromolecule_id="7">
                <name>PC4 and SFRS1-interacting protein</name>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.057961043</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEG
LWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETE
EAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKE
EDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEE
QMETEQQNKDEGKKPEVKKVEKKRETSMDSRLQRIHAEIKNSLKIDNLDVNRCIEALDELASLQVTMQQAQKHTEMITTL
KKIRRFKVSQVIMEKSTMLYNKFKNMFLVGEGDSVITQVLNKSLAEQRQHEEANKTKDQGKKGPNKKLEKEQTGSKTLNG
GSDAQDGNQPQHNGESNEDSKDNHEASTKK</string>
                    <external_references type="UNIPROTKB">O75475</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
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            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
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            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">30.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_magnification>165000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <zemlin_tableau/>
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Bioquantum</name>
                            <slit_width units="eV">10</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <digitization_details/>
                            <number_grids_imaged>1</number_grids_imaged>
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                            <average_electron_dose_per_image units="e/Å^2">55.0</average_electron_dose_per_image>
                        </image_recording>
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            </microscopy_list>
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                <image_recording_id>1</image_recording_id>
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                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">7.0</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
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                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
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