2021-08-25 OBS 2020-05-18 PDBe PDBe 2020-10-21 2020-10-21 2021-08-25 CryoEM structure of simian T-cell lymphotropic virus intasome in complex with PP2A regulatory subunit B56 gamma Barski M, Hodakova Z, Nans A, Cherepanov P, Maertens GN 6z2y Barski MS, Minnell JJ, Hodakova Z, Pye VE, Nans A, Cherepanov P, Maertens GN Cryo-EM structure of the deltaretroviral intasome in complex with the PP2A regulatory subunit B56 gamma. Nat Commun 11 5043 5043 2020 33028863 doi:10.1038/s41467-020-18874-y 2041-1723 emd_11052.map.gz Image stored as Reals 137 76 113 82.0 110.0 94.0 218.0 185.0 206.0 113 137 76 123.170006 149.33 82.840004 90.0 90.0 90.0 Z Y X -2.466581 3.8886082 -7.282095e-14 0.24949118 1
1.09 1.09 1.09 0.15 Density Modified map generated using Resolve in Phenix. Contour 0.15, step 1 in Chimera; 3 rmsd in Coot 9 Complex of STLV-1 MarB43 integrase with nascent viral DNA and the human PP2A B56 subunit
Sample composition and source have been described in "macromolecules"
protein Complex of STLV-1 MarB43 integrase with nascent viral DNA and the human PP2A B56 subunit
Sample composition and source have been described in "macromolecules"
false protein Pol Protein
Pol Protein
Simian T-lymphotropic virus 1 false Escherichia coli protein PC4 and SFRS1-interacting protein,Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
PC4 and SFRS1-interacting protein,Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Homo sapiens false Escherichia coli protein DNA
DNA
Simian T-lymphotropic virus 1 false synthetic construct protein Pol protein 0.033885504 Simian T-lymphotropic virus 1 4 false Escherichia coli protein PC4 and SFRS1-interacting protein,Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform 0.08039468
fusion construct containing human LEDGF (residues 1-324 thus without the IBD domain) (gene PSIP1; O75475)) and human B56gammma (residues 11-380) regulatory subunit of PP2A (gene PPP2R5C) (no space for these details below),fusion construct containing human LEDGF (residues 1-324 thus without the IBD domain) (gene PSIP1; O75475)) and human B56gammma (residues 11-380) regulatory subunit of PP2A (gene PPP2R5C) (no space for these details below)
Homo sapiens 2 false Escherichia coli nucleic-acid DNA (5'-D(*AP*CP*TP*GP*TP*GP*TP*TP*TP*GP*GP*CP*GP*CP*TP*TP*CP*TP*CP*TP*C)-3') 0.009221908999999999 Simian T-lymphotropic virus 1 false (DA)(DC)(DT)(DG)(DT)(DG)(DT)(DT)(DT)(DG)(DG)(DC)(DG)(DC)(DT)(DT)(DC)(DT)(DC)(DT) (DC)(DC)(DC)(DG)(DG)(DA)(DG)(DA)(DG)(DA) DNA OTHER nucleic-acid DNA (5'-D(*GP*AP*GP*AP*GP*AP*AP*GP*CP*GP*CP*CP*AP*AP*AP*CP*AP*CP*A)-3') 0.008593559999999998 Simian T-lymphotropic virus 1 false (DT)(DC)(DT)(DC)(DT)(DC)(DC)(DG)(DG)(DG)(DA)(DG)(DA)(DG)(DA)(DA)(DG)(DC)(DG)(DC) (DC)(DA)(DA)(DA)(DC)(DA)(DC)(DA) DNA OTHER ligand ZINC ION 6.5409e-05 4 false ETHANE 95 295.15 FEI VITROBOT MARK IV ETHANE 95 295.15 FEI VITROBOT MARK IV FIELD EMISSION GUN 50.4 BRIGHT FIELD FLOOD BEAM FEI FALCON III (4k x 4k) FEI TITAN KRIOS FEI TITAN KRIOS AUTOGRID HOLDER 300 6QBW 6TOQ 6TJU 5JJA A A C A rigid body 31.31 REAL 31.31 REAL
Baverage protein=31.31; DNA=49.12; zinc=38.96
 particle 0.76 6.0 singleParticle RELION 3.34 FSC 0.143 CUT-OFF
3D reconstruction, including Bayesian particle polishing, CTF and beam tilt refinement, as implemented in Relion-3.1, resulted in the final map with minimal anisotropy; 3D-FSC sphericity index of the final map was 0.967.
 C2 161921