2021-11-22 OBS PDBe PDBe PDBe 2019-08-02 2021-12-01 2021-12-01 2021-11-22 CryoEM structure of the external needle filament from the Salmonella typhimurium type-3 secretion system Kotov V Marlovits TC Goessweiner-Mohr N Kotov V Brunner MJ Mayr J Wald J Kuhlen L Miletic S Vesper O Lugmayr W Wagner S DiMaio F Lea S Marlovits TC Structural control for the coordinated assembly into functional pathogenic type-3 secretion systems To Be Published 0353 EMD-10167 associated EM volume
CryoEM structure of the external needle filament from the Salmonella typhimurium type-3 secretion system
 6sfs FULLOVERLAP T3SS complex T3SS complex 0 1
isolated from wild-type strain (SB905)
Salmonella typhimurium PrgI Salmonella typhimurium 0.008864868 27 LEVO MATPWSGYLDDVSAKFDTGVDNLQTQVTEALDKLAAKPSDPALLAAYQSKLSEYNLYRNAQSNTVKVFKDIDAAIIQNFR helical particle 3 8.0 10.0 Tris Tris 500.0 NaCl Sodium chloride 5.0 EDTA Ethylenediaminetetraacetic acid 0.1 LDAO Lauryldimethylamine oxide ETHANE-PROPANE 100 FEI VITROBOT MARK IV
monodisperse
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 GATAN K2 SUMMIT (4k x 4k) COUNTING 1-25 31.5 1 4.4 63.3 C1 2.72 FSC 0.143 CUT-OFF 133516
Soft-edged cylinder
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