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    <admin>
        <lastUpdate>2021-11-22</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2019-06-19</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2021-12-01</headerReleaseDate>
        <obsoletedDate>2021-12-01</obsoletedDate>
        <title>Nuclear protein complex protein 2 only</title>
        <authors>Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva C, Zaher MS, Blanco FJ, Hamdan SM, De Biasio A</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva C, Zaher MS, Blanco FJ, Hamdan SM, De Biasio A</authors>
                <articleTitle>Structure of the human lagging strand DNA polymerase (Pol delta) holoenzyme</articleTitle>
                <journal>To be published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>360</numRows>
            <numColumns>360</numColumns>
            <numSections>360</numSections>
        </dimensions>
        <origin>
            <originRow>0.0</originRow>
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        <limit>
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        </limit>
        <spacing>
            <spacingRow>360</spacingRow>
            <spacingCol>360</spacingCol>
            <spacingSec>360</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">388.80002</cellA>
            <cellB units="A">388.80002</cellB>
            <cellC units="A">388.80002</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.05503358</minimum>
            <maximum>0.070285015</maximum>
            <average>2.2825967e-05</average>
            <std>0.0011505451</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
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            <pixelX units="A">1.08</pixelX>
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        </pixelSpacing>
        <contourLevel source="author">0.01</contourLevel>
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    <sample>
        <numComponents>4</numComponents>
        <name>Replicative state</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Replicative state</sciName>
                <protein>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource />
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Proliferating cell nuclear antigen</sciName>
                <protein>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>nucleic-acid</entry>
                <sciName>DNA Template</sciName>
                <nucleic-acid>
                    <sciSpeciesName ncbiTaxId="32644">unidentified</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                    <sequence>CTGCACGAATTAAGCAATTCGTAATCATGGTCATAGCT</sequence>
                    <class>DNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>nucleic-acid</entry>
                <sciName>DNA Primer</sciName>
                <nucleic-acid>
                    <sciSpeciesName ncbiTaxId="32644">unidentified</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                    <sequence>AGCTATGACCATGATTACGAATTG(DOC)</sequence>
                    <class>DNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>100</humidity>
            <temperature units="Kelvin">277</temperature>
            <instrument>FEI VITROBOT MARK IV</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">35.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <nominalDefocusMin units="nm">400.0</nominalDefocusMin>
            <nominalDefocusMax units="nm">600.0</nominalDefocusMax>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON III (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <calibratedMagnification>130000.0</calibratedMagnification>
            <temperatureMin units="Kelvin">77.0</temperatureMin>
            <temperatureMax units="Kelvin">77.0</temperatureMax>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
            <nominalMagnification>75000.0</nominalMagnification>
        </imaging>
        <imageAcquisition>
            <numDigitalImages>3575</numDigitalImages>
            <samplingSize units="microns">14.0</samplingSize>
            <details>75 fractions per image</details>
        </imageAcquisition>
        <fitting>
            <pdbEntryIdList />
            <refProtocol>rigid body</refProtocol>
        </fitting>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">0.355</specimenConc>
            <buffer>
                <ph>7.5</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <resolutionByAuthor>6.9</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C1</appliedSymmetry>
            <numProjections>149306</numProjections>
        </singleParticle>
    </processing>
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