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    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2019-01-14</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>The cryo-EM structure of tetrameric ArnA</title>
        <authors>Yang M, Gehring K</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Yang M, Chen YS, Ichikawa M, Calles D, Basu K, Bui KH, Ortega J, Gehring K</authors>
                <articleTitle>Cryo-EM structures of ArnA features unexpected oligomerizations and domain movements</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
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        <dataType>Image stored as Reals</dataType>
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            <numColumns>210</numColumns>
            <numSections>210</numSections>
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        <spacing>
            <spacingRow>210</spacingRow>
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            <spacingSec>210</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">225.32999</cellA>
            <cellB units="A">225.32999</cellB>
            <cellC units="A">225.32999</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
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            <maximum>0.15510279</maximum>
            <average>0.0025752522</average>
            <std>0.011269629</std>
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        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
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        <contourLevel source="author">0.0339</contourLevel>
        <annotationDetails>Cryo-EM structure of tetrameric ArnA</annotationDetails>
    </map>
    <sample>
        <numComponents>1</numComponents>
        <name>ArnA</name>
        <details>Full length ArnA from E.coli</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>ArnA</sciName>
                <molWtExp units="MDa">0.44</molWtExp>
                <details>Full length ArnA from E.coli</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="668369">Escherichia coli DH5[alpha]</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>100</humidity>
            <temperature units="Kelvin">298</temperature>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">35.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>SPOT SCAN</illuminationMode>
            <detector>FEI FALCON II (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">1.2</specimenConc>
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    <processing>
        <method>singleParticle</method>
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            <resolutionByAuthor>8.7</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C1</appliedSymmetry>
            <numProjections>38127</numProjections>
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