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        <title>Stringent Response Control by a Bifunctional RelA enyzme in the Presence and Absence of the Ribosome</title>
        <authors>Wilson DN, Abdelshahid M</authors>
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                <articleTitle>Stringent Response Control by a Bifunctional RelA enyzme in the Presence and Absence of the Ribosome</articleTitle>
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                    <sequence>UUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAUGAAGGACGGGACGAACACCGAUAUGCUUCGGG GAGCUGUAAGCAAGCUUUGAUCCGGAGAUUUCCGAAUGGGGAAACCCACCACUCGUAAUGGAGUGGUAUCCAUAUCUGAA UUCAUAGGAUAUGAGAAGGCAGACCCGGGGAACUGAAACAUCUAAGUACCCGGAGGAAGAGAAAGCAAAUGCGAUUCCCU GAGUAGCGGCGAGCGAAACGGGAUCAGCCCAAACCAAGAGGCUUGCCUCUUGGGGUUGUAGGACACUCUGUACGGAGUUA CAAAGGAACGAGGUAGAUGAAGAGGUCUGGAAAGGCCCGCCAUAGGAGGUAACAGCCCUGUAGUCAAAACUUCGUUCUCU CCUGAGUGGAUCCUGAGUACGGCGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAUACUC CCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAGAGAUCCUGAAAC CGUGUGCCUACAAGUAGUCAGAGCCCGUUAACGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUCUCG UGCAAGGUUAAGCAGAAGAUGCGGAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGCAUGAGUACGUGGUCGUAGACCCGA AACCAGGUGAUCUACCCAUGUCCAGGGUGAAGUUCAGGUAACACUGAAUGGAGGCCCGAACCCACGCACGUUGAAAAGUG CGGGGAUGAGGUGUGGGUAGGGGUGAAAUGCCAAUCGAACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUA GCCUCAAGGUAAGAGUCUUGGAGGUAGAGCACUGAUUGGACUAGGGGCCCCUACCGGGUUACCGAAUUCAGUCAAACUCC GAAUGCCAAUGACUUAUCCUUGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAAAGGGAAACAGCCCAGACCGCCA GCUAAGGUCCCAAAGUAUACGUUAAGUGGAAAAGGAUGUGGAGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGCAGCC ACCAUUUAAAGAGUGCGUAAUAGCUCACUGGUCGAGUGACUCUGCGCCGAAAAUGUACCGGGGCUAAACGUAUCACCGAA GCUGCGGACUGUUCUUCGAACAGUGGUAGGAGAGCGUUCUAAGGGCUGUGAAGCCAGACCGGAAGGACUGGUGGAGCGCU UAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAGGGGUGAGAAUCCCCUCCACCGAAUGCCUAAGGUUUCCUGAGGAAG GCUCGUCCGCUCAGGGUUAGUCGGGACCUAAGCCGAGGCCGAAAGGCGUAGGCGAUGGACAACAGGUUGAUAUUCCUGUA CCACCUCCUCACCAUUUGAGCAAUGGGGGGACGCAGGAGGAUAGGGUAAGCGCGGUAUUGGAUAUCCGCGUCCAAGCAGU UAGGCUGGGAAAUAGGCAAAUCCGUUUCCCAUAAGCCUGAGCUGUGAUGGCGAGCGAAAUAUAGUAGCGAAGUUCCUGAU UCCACACUGCCAAGAAAAGCCUCUAGCGAGGUGAGAGGUGCCCGUACCGCAAACCGACACAGGUAGGCGAGGAGAGAAUC CUAAGGUGAUCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCGGGAGAAGGGGUGCUCUGUUAGG GUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGCGACUGUUUAGCAAAAACACAGGUCUCUGCGAAGCCGUAAGGC GAAGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGCGCUUAGCGUAAGCGAAGGUGCGAAUUGAAGCC CCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGG CGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGG ACGGAAAGACCCCGUGGAGCUUUACUGCAGCCUGAUAUUGAAUGUUGGUACAGCUUGUACAGGAUAGGUAGGAGCCUUGG AAACCGGAGCGCCAGCUUCGGUGGAGGCAUCGGUGGGAUACUACCCUGGCUGUAUUGACCUUCUAACCCGCCGCCCUUAU CGGGCGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAGGUUC CCUCAGAAUGGUUGGAAAUCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGGGA CGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAACA GGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGU CGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCU AUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACGCACCGCUGGUGUACCA GUUGUUCUGCCAAGGGCAUCGCUGGGUAGCUAUGUGCGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCU CAAGAUGAGAUUUCCCAUUCCGCAAGGAAGUAAGAUCCCUGAAAGAUGAUCAGGUUGAUAGGUCUGAGGUGGAAGUGUGG CGACACAUGGAGCUGACAGAUACUAAUCGAUCGAGGACUUAAC</sequence>
                    <class>RNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
            <sampleComponent componentID="34">
                <entry>nucleic-acid</entry>
                <sciName>5S rRNA</sciName>
                <molWtTheo units="MDa">0.03615752</molWtTheo>
                <nucleic-acid>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                    <sequence>UGGUGGCGAUAGCGAAGAGGUCACACCCGUUCCCAUACCGAACACGGAAGUUAAGCUCUUCAGCGCCGAUGGUAGUCGGG GGUUUCCCCCUGUGAGAGUAGGACGCCGCCAA</sequence>
                    <class>RNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
            <sampleComponent componentID="35">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L2</sciName>
                <molWtTheo units="MDa">0.030074749999999997</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="36">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L3</sciName>
                <molWtTheo units="MDa">0.022462973</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="37">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L4</sciName>
                <molWtTheo units="MDa">0.022222675999999997</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="38">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L5</sciName>
                <molWtTheo units="MDa">0.020046370999999997</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="39">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L6</sciName>
                <molWtTheo units="MDa">0.019124855</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="40">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L10</sciName>
                <molWtTheo units="MDa">0.013607532999999998</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="41">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L11</sciName>
                <molWtTheo units="MDa">0.014048275999999998</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="42">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L13</sciName>
                <molWtTheo units="MDa">0.01601761</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="43">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L14</sciName>
                <molWtTheo units="MDa">0.013175288</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="44">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L15</sciName>
                <molWtTheo units="MDa">0.015410693999999999</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="45">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L16</sciName>
                <molWtTheo units="MDa">0.015619512</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="46">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L17</sciName>
                <molWtTheo units="MDa">0.013643609</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="47">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L18</sciName>
                <molWtTheo units="MDa">0.012993828999999998</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="48">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L19</sciName>
                <molWtTheo units="MDa">0.013285655</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="49">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L20</sciName>
                <molWtTheo units="MDa">0.013408813</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="50">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L21</sciName>
                <molWtTheo units="MDa">0.011164886</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="51">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L22</sciName>
                <molWtTheo units="MDa">0.012035066</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="52">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L23</sciName>
                <molWtTheo units="MDa">0.010778621999999998</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="53">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L24</sciName>
                <molWtTheo units="MDa">0.010790656999999999</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="54">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L27</sciName>
                <molWtTheo units="MDa">0.008956143</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="55">
                <entry>protein</entry>
                <sciName>GTP pyrophosphokinase</sciName>
                <molWtTheo units="MDa">0.039164523</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis (strain 168)</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="56">
                <entry>nucleic-acid</entry>
                <sciName>E-tRNA</sciName>
                <molWtTheo units="MDa">0.024140334</molWtTheo>
                <nucleic-acid>
                    <sciSpeciesName ncbiTaxId="562">Escherichia coli</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                    <sequence>CCCGGAUAGCUCAGUCGGUAGAGCAGGGGAUUGAAAAUCCCCGUGUCCUUGGUUCGAUUCCGAGUCCGGGCACCA</sequence>
                    <class>RNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
            <sampleComponent componentID="57">
                <entry>nucleic-acid</entry>
                <sciName>P-tRNA</sciName>
                <molWtTheo units="MDa">0.024747745999999998</molWtTheo>
                <nucleic-acid>
                    <sciSpeciesName ncbiTaxId="562">Escherichia coli</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                    <sequence>CGCGGGGUUGAGCAGCCUGGUAGCUCGUCGGGCUCAUAACCCGAAGAUCGUCGGUUCAAAUCCGGCCCCCGCAACCA</sequence>
                    <class>RNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
            <sampleComponent componentID="58">
                <entry>nucleic-acid</entry>
                <sciName>A/R-tRNA</sciName>
                <molWtTheo units="MDa">0.024486523</molWtTheo>
                <nucleic-acid>
                    <sciSpeciesName ncbiTaxId="562">Escherichia coli</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                    <sequence>GCCCGGAUAGCUCAGUUGGUAGAGCAGGGGAUUGAAAAUCCCCGUGUCCUUGGUUCGAUUCCGAGUCCGGGCACCA</sequence>
                    <class>RNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">2.4</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON II (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>7.2</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <resolutionByAuthor>4.5</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <numProjections>650054</numProjections>
        </singleParticle>
    </processing>
</emdEntry>