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    <admin>
        <lastUpdate>2019-05-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-06-18</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2018-08-22</headerReleaseDate>
        <obsoletedDate>2019-05-01</obsoletedDate>
        <title>Cryo-EM map of yeast Tsr1-TAP S20Dloop pre-40S particles, class C2-S20Dloop</title>
        <authors>Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Gleizes PE, Omanic H, Kressler D, Plisson-Chastang C, Pertschy B</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Gleizes PE, Omanic H, Kressler D, Plisson-Chastang C, Pertschy B</authors>
                <articleTitle>Conformational proofreading of distant 40S subunit maturation events by a long-range communication mechanism</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>360</numRows>
            <numColumns>360</numColumns>
            <numSections>360</numSections>
        </dimensions>
        <origin>
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        <limit>
            <limitRow>359.0</limitRow>
            <limitCol>359.0</limitCol>
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        </limit>
        <spacing>
            <spacingRow>360</spacingRow>
            <spacingCol>360</spacingCol>
            <spacingSec>360</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">446.4</cellA>
            <cellB units="A">446.4</cellB>
            <cellC units="A">446.4</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.012680445</minimum>
            <maximum>0.07712485</maximum>
            <average>0.00046947</average>
            <std>0.004399702</std>
        </statistics>
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        <details />
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            <pixelX units="A">1.24</pixelX>
            <pixelY units="A">1.24</pixelY>
            <pixelZ units="A">1.24</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.012</contourLevel>
        <annotationDetails>Cryo-EM map of yeast pre-40S particles purified with Tsr1-TAP as bait, in Rps20 Delta loop mutant conditions (deletion of Rps20 aa 68-78). Class C2-S20Dloop.</annotationDetails>
    </map>
    <sample>
        <numComponents>1</numComponents>
        <name>Yeast pre-40S particle, purified using Tsr1-TAP as bait, in mutant conditions (deletion of aa 68-78 of Rps20)</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Yeast pre-40S particle, purified using Tsr1-TAP as bait, in mutant conditions (deletion of aa 68-78 of Rps20)</sciName>
                <molWtTheo units="MDa">1.2</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="4932">Saccharomyces cerevisiae</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="4932">Saccharomyces cerevisiae</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>90</humidity>
            <temperature units="Kelvin">293</temperature>
            <instrument>LEICA EM GP</instrument>
            <details />
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">2.275</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON III (4k x 4k)</detector>
            <microscope>FEI TALOS ARCTICA</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">200</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">0.03</specimenConc>
            <buffer>
                <ph>7.4</ph>
            </buffer>
            <specimenSupportDetails>30 mA</specimenSupportDetails>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <algorithm>EXACT BACK PROJECTION</algorithm>
            <software>RELION</software>
            <resolutionByAuthor>6.6</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C1</appliedSymmetry>
            <numProjections>35458</numProjections>
        </singleParticle>
    </processing>
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