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    <admin>
        <current_status>
            <date>2019-05-01</date>
            <code>OBS</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2018-06-16</deposition>
            <header_release>2018-08-22</header_release>
            <obsolete>2019-05-01</obsolete>
            <update>2019-05-01</update>
        </key_dates>
        <title>Cryo-EM map of yeast Tsr1-TAP WT pre-40S particles, class C2-WT</title>
        <authors_list>
            <author>Mitterer V</author>
            <author>Shayan R</author>
            <author>Ferreira-Cerca S</author>
            <author>Murat G</author>
            <author>Enne T</author>
            <author>Rinaldi D</author>
            <author>Weigl S</author>
            <author>Gleizes PE</author>
            <author>Omanic H</author>
            <author>Kressler D</author>
            <author>Plisson-Chastang C</author>
            <author>Pertschy B</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Mitterer V</author>
                    <author order="2">Shayan R</author>
                    <author order="3">Ferreira-Cerca S</author>
                    <author order="4">Murat G</author>
                    <author order="5">Enne T</author>
                    <author order="6">Rinaldi D</author>
                    <author order="7">Weigl S</author>
                    <author order="8">Gleizes PE</author>
                    <author order="9">Omanic H</author>
                    <author order="10">Kressler D</author>
                    <author order="11">Plisson-Chastang C</author>
                    <author order="12">Pertschy B</author>
                    <title>Conformational proofreading of distant 40S subunit maturation events by a long-range communication mechanism</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-0053</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-0059</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
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        <name>yeast pre-40S particle, purified with Tsr1 as bait in WT conditions, Class C2-WT</name>
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            <complex_supramolecule supramolecule_id="1">
                <name>yeast pre-40S particle, purified with Tsr1 as bait in WT conditions, Class C2-WT</name>
                <parent>0</parent>
                <molecular_weight>
                    <theoretical units="MDa">1.2</theoretical>
                </molecular_weight>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>yeast pre-40S particle, purified with Tsr1 as bait, Class C2-WT</name>
                <parent>1</parent>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>yeast pre-40S particle, purified with Tsr1 as bait, Class C1-WT</name>
                <parent>1</parent>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="4932">Saccharomyces cerevisiae</recombinant_organism>
                </recombinant_expression>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.03</concentration>
                    <buffer>
                        <ph>7.4</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">95</chamber_humidity>
                        <chamber_temperature units="K">293</chamber_temperature>
                        <instrument>LEICA EM GP</instrument>
                        <details></details>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON II (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">6.51</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>CTFFIND</name>
                            <version>4</version>
                        </software>
                    </software_list>
                </ctf_correction>
                <startup_model type_of_model="PDB ENTRY">
                    <pdb_model>
                        <pdb_id>4V88</pdb_id>
                    </pdb_model>
                </startup_model>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">6.6</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                    <number_images_used>11768</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                </final_angle_assignment>
                <final_three_d_classification>
                    <software_list>
                        <software>
                            <name>RELION</name>
                        </software>
                    </software_list>
                </final_three_d_classification>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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        <symmetry>
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            <a units="Å">403.2</a>
            <b units="Å">403.2</b>
            <c units="Å">403.2</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
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            <average>0.00037425346</average>
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        <label>::::EMDATABANK.org::::EMD-0059::::                                              </label>
        <annotation_details>Cryo-EM map of yeast Tsr1-TAP WT particles, class C2</annotation_details>
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                <label>::::EMDATABANK.org::::EMD-0059::::                                              </label>
                <annotation_details>Cryo-EM map of yeast Tsr1-TAP WT particles, class C2, half-map 1</annotation_details>
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                <label>::::EMDATABANK.org::::EMD-0059::::                                              </label>
                <annotation_details>Cryo-EM map of yeast Tsr1-TAP WT particles, class C2, half-map 1</annotation_details>
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