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    <admin>
        <lastUpdate>2021-11-22</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-05-07</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2021-12-01</headerReleaseDate>
        <obsoletedDate>2021-12-01</obsoletedDate>
        <title>Subtomogram averaging of nucleosome particles in chromatin plates from metaphase chromosomes</title>
        <authors>Chicano A, Crosas E, Engel BD, Melero R, Oton J, Daban JR</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Chicano A, Crosas E, Engel BD, Melero R, Oton J, Daban JR</authors>
                <articleTitle>Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
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            <numColumns>50</numColumns>
            <numSections>50</numSections>
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            <spacingRow>50</spacingRow>
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            <spacingSec>50</spacingSec>
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            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
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        <statistics>
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            <maximum>0.17914577</maximum>
            <average>5.0426933e-12</average>
            <std>0.05552994</std>
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        <details />
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        <annotationDetails>Structure of in-plate particles</annotationDetails>
    </map>
    <sample>
        <numComponents>1</numComponents>
        <name>Chromatin plates from metaphase chromosomes</name>
        <details>Nucleosome particles in the plates</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Chromatin plates from metaphase chromosomes</sciName>
                <details>Nucleosome particles in the plates</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="9606">Homo sapiens</sciSpeciesName>
                    <sciSpeciesStrain>HeLa</sciSpeciesStrain>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <natSource>
                        <organelle>Metaphase chromosomes</organelle>
                    </natSource>
                    <engSource />
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>OTHER</cryogenName>
            <instrument>FEI VITROBOT MARK III</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
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            <energyFilter>GIF 2002</energyFilter>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <nominalDefocusMax units="nm">0.5</nominalDefocusMax>
            <illuminationMode>OTHER</illuminationMode>
            <detector>GATAN K2 SUMMIT (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
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            <specimenState>particle</specimenState>
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                <ph>7.2</ph>
                <details>5 mM Pipes (pH 7.2), 5 mM NaCl, and 5 mM MgCl2</details>
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        </specimenPreparation>
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        <method>subtomogramAveraging</method>
        <reconstruction>
            <algorithm>FOURIER SPACE</algorithm>
            <resolutionByAuthor>30.0</resolutionByAuthor>
            <resolutionMethod>OTHER</resolutionMethod>
            <details>MonoRes</details>
        </reconstruction>
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        </subtomogramAveraging>
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